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Exploration of core endophytic bacteria from different organs of diploid Musa balbisiana and triploid Musa acuminata
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 055, Issue 5, November 2021- December 2021, Pages 787-794
ISSN: 2452-316X(0075-5192)
DOI: doi.org/10.34044/j.anres.2021.55.5.09
Triastuti Rahayua, Yekti Asih Purwestrib, Siti Subandiyahc, Ahmad Suparmind, Donny Widiantod,*
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aGraduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia, Biology Education Study Program, Faculty of Teacher Training and Education, Universitas Muhammadiyah Surakarta, Sukoharjo 57162, Indonesia
bResearch Center for Biotechnology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
cDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
dDepartment of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
*Corresponding author, e-mail: donny@ugm.ac.id
Abstract
Musa balbisiana Colla cv. Kluthuk (BB) is extensively cultivated because it is relatively resistant to biotic and abiotic stresses, while there have been difficulties regarding the cultivation of the less-tolerant cultivar Musa acuminata cv. Ambon (AAA). Furthermore, the composition of banana genomes has been reported to play an important role in resistance. Even though their microbiome is involved also in plant adaptability and survival, no analysis has been performed to compare the bacterial composition and its role between resistant and susceptible cultivars. Hence, this study determined the core endophytic bacteria in the Kluthuk and Ambon cultivars growing in loamy sand (LM) and silt loam (SL) soil types. The banana organs (roots, corm and petiole) were subjected to 16S amplicon sequencing. The data obtained showed that the Proteobacteria, Actinobacteria, Firmicutes and Acidobacteria dominated all organs in both cultivars at the phyla level, while Bacillus, Gaiella and Sphingomonas dominated at the genus level. Interestingly, cv. Ambon had a lower number of core endophytic bacteria compared to cv. Kluthuk. Pseudomonas was discovered pre-eminently in the corms and petioles, followed by Bacillus in all organs of Kluthuk. Conversely, the same genera were depleted in Ambon, and instead, high levels of Brevibacillus and Acinetobacter were detected mainly in the roots. This was the first examination of the core endophytic bacterial distribution between these cultivars, which is also necessary for further banana resistance development through amendment of the bacteria.
Keywords
Core endophytic bacteria, Musa acuminata, Musa balbisiana
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