Application of elicitors (jasmonic acid, salicylic acid and nanosheet) for in vitro growth and biochemical properties of Siam tulip (Curcuma alismatifolia cv. Maejo Impress)

Orada Chumphukam; Yupha Saeyang; Pimpakay Baiya; Nutchanan Duangkon; Sukalya Poothong

Application of elicitors (jasmonic acid, salicylic acid and nanosheet) for in vitro growth and biochemical properties of Siam tulip (Curcuma alismatifolia cv. Maejo Impress)

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 057, Issue 6, November 2023- December 2023, Pages 963-970

ISSN: 2452-316X(0075-5192)

DOI: doi.org/10.34044/j.anres.2023.57.6.06

Orada Chumphukam^a, Yupha Saeyang^a, Pimpakay Baiya^a, Nutchanan Duangkon^b, Sukalya Poothong^b,*

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^aSchool of Medicinal Science, University of Phayao, Phayao 56000, Thailand

^bSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand

^*Corresponding author, e-mail: sukalya.po@up.ac.th

Abstract

Importance of the work: The Siam tulip (Curcuma alismatifolia) is an economic crop with the potential for producing bioactive compound through plant tissue culture. Objectives: To investigate the effects of jasmonic acid, salicylic acid and two-dimensional nanomaterials (nanosheets) on the in vitro growth of C. alismatifolia cv. Maejo Impress and its phytochemical properties. Materials & Methods: Two types of elicitors were tested at three concentrations and they were combined with a nanosheet. The control group utilized a basal Murashige and Skoog medium. The experiment units were assigned following a completely randomized design. After 6 wk of culture, growth, total phenolic content and antioxidant activity of the Siam tulip cv. Maejo Impress were studied. Results: The addition of elicitors had no significant effect on the height of explants and new shoots, shoot multiplication and leaf colour. However, the Siam tulip cultured with 4 mg/L and 6 mg/L jasmonic acid (JA) had the highest (p < 0.05) total phenolic content and antioxidant activity compared to those cultured without elicitors. Main finding: The optimal type and concentrations of elicitors were 4–6 mg/L JA for improving the accumulation of bioactive compounds in the micropropagation of C. alismatifolia cv. Maejo Impress without inducing shoot necrosis or death.

Keywords

Curcuma alismatifolia, Elicitors, Jasmonic acid, Nano sheets, Salicylic acid

Agriculture and Natural Resources (ANRES)

Application of elicitors (jasmonic acid, salicylic acid and nanosheet) for in vitro growth and biochemical properties of Siam tulip (Curcuma alismatifolia cv. Maejo Impress)

Abstract

Keywords

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