Agriculture and Natural Resources (ANRES)

The improvement of the control efficiency of plant extracts on Spodoptera frugiperda (Lepidoptera: Noctuoidea) by mixture formula development
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume Inpress, Issue i
ISSN: 2452-316X(0075-5192)
Nutchaya Kumrungseea, Nattarat Tipsutb, Lalita Pimsawangc, Wanchai Pluempaupatc, Vasakorn Bullangpotib,*
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aAnimal toxicology and physiology specialty research unit, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900 Thailand. Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi 12110 Thailand.
bAnimal toxicology and physiology specialty research unit, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900 Thailand
cDepartment of Chemistry, Center of Excellence for Innovation in Chemistry and Special Research Unit for Advanced Magnetic Resonance, Faculty of Science, Kasetsart University, Bangkok, 10900 Thailand
*Corresponding author, e-mail: fscivkb@ku.ac.th
Abstract
Importance of this work: This research has the goal of improving control efficiency against Spodoptera frugiperda via the development of mixed plant extracts. Objectives: Our research aimed to assess the insecticidal potential of various plant crude extracts against S. frugiperda larvae for the future development of effective insecticide products. Materials and methods: The insecticidal potential of various crude extracts from Cyperus rotundus mixed with Piper retrofractum against S. frugiperda larvae was investigated. The acute effects of binary mixtures of various compounds at concentrations equal to the LD30, LD20, and LD10 values of each compound were determined. Results: The best formulations were P. retrofractum hexane crude extract (at a dose of 10.57 ppm) and C. rotundus dichloromethane extract (at a dose of 2504 ppm), which caused mortality rates of up to 98% compared with 20% when individual extracts were used. Main finding: This mixture formulation allows for the use of smaller amounts of plant substances, resulting in lower production costs. This information is necessary for the future development of effective insecticide products.
Keywords
Detoxification enzymes, Plant extracts, Spodoptera sp
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